Abstract
Enterococci with gentamicin MICs of 256 to 1,024 micrograms/mL were evaluated for susceptibility to ampicillin plus gentamicin synergism. Sixteen of eighteen enterococcal isolates were not susceptible to synergistic killing by ampicillin plus gentamicin; 11 possessed aac(6')-aph(2"), and 4 possessed aph(2")-Ic. A gentamicin MIC of 512 or 1,024 micrograms/mL predicted lack of ampicillin/gentamicin synergism, but a gentamicin MIC of 256 micrograms/mL did not. For six enterococcal strains possessing the gentamicin-resistance gene aph(2")-Ic, ampicillin plus dibekacin, ampicillin plus netilmicin, and ampicillin plus amikacin produced synergistic killing in five, three, and two strains, respectively.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amikacin / pharmacology
-
Amikacin / therapeutic use
-
Ampicillin / pharmacology*
-
Ampicillin / therapeutic use
-
Animals
-
DNA Primers / chemistry
-
DNA Probes / chemistry
-
DNA Restriction Enzymes
-
Dibekacin / pharmacology
-
Dibekacin / therapeutic use
-
Drug Resistance, Microbial / genetics
-
Drug Synergism
-
Electrophoresis, Agar Gel
-
Enterococcus faecium / drug effects*
-
Enterococcus faecium / genetics
-
Gentamicins / pharmacology*
-
Gentamicins / therapeutic use
-
Gram-Positive Bacterial Infections / drug therapy
-
Gram-Positive Bacterial Infections / microbiology
-
Humans
-
Microbial Sensitivity Tests
-
Netilmicin / pharmacology
-
Netilmicin / therapeutic use
-
Penicillins / pharmacology*
-
Penicillins / therapeutic use
-
Polymerase Chain Reaction
Substances
-
DNA Primers
-
DNA Probes
-
Gentamicins
-
Penicillins
-
Dibekacin
-
Netilmicin
-
Ampicillin
-
Amikacin
-
DNA Restriction Enzymes