Abstract
Crude theaflavin was extracted from black tea and then fractionated by HPLC into five components (initial peaks (IP), TF1, TF2A, TF2B, and TF3). The crude extract and the various fractions of theaflavin were collected and tested, individually and in combination, for antirotaviral activity. The mean effective concentration (EC50) was calculated and compared. Activity varied from the most active being the uncharacterized theaflavin-like initial peaks (IP) with an EC50 of 0.125 microgram/ml to the least active being theaflavin-3 monogallate (TF2A) with an EC50 of 251.39 micrograms/ ml. The combination of TF1 + TF2A + TF2B + TF3 was more active than the sum of the activities of these four fractions individually, indicating synergism among the peaks. Only the crude extract was assayed for activity against coronavirus; the EC50 was 34.7 micrograms/ml.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / chemistry
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Antiviral Agents / isolation & purification*
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Antiviral Agents / pharmacology*
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Biflavonoids*
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Catechin*
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Cattle
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Cattle Diseases / drug therapy*
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Cell Line
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Chelating Agents / chemistry
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Chelating Agents / isolation & purification
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Chelating Agents / pharmacology
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Chromatography, High Pressure Liquid
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Coronavirus Infections / drug therapy
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Coronavirus Infections / veterinary*
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Coronavirus, Bovine / drug effects*
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Coronavirus, Bovine / physiology
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Gallic Acid / analogs & derivatives
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Gallic Acid / chemistry
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Gallic Acid / isolation & purification
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Gallic Acid / pharmacology
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Models, Molecular
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Molecular Conformation
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Rotavirus / drug effects*
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Rotavirus / physiology
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Rotavirus Infections / drug therapy
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Rotavirus Infections / veterinary*
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Tea / chemistry*
Substances
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Antiviral Agents
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Biflavonoids
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Chelating Agents
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Tea
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theaflavin
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theaflavine gallate
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Gallic Acid
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Catechin