Abstract
Bordetella pertussis expresses a bvg-regulated 95-kDa protein, Vag8, encoded by vag-8. Southern blot analysis indicates that strains of Bordetella bronchiseptica and Bordetella parapertussis have DNA homologous to vag-8. Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of B. pertussis and B. bronchiseptica but not B. parapertussis. A 95-kDa protein-negative derivative of B. pertussis 18323 containing a deletion of vag-8 colonized mice as efficiently as the parent B. pertussis strain in a mouse aerosol model of pertussis.
MeSH terms
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Alkaline Phosphatase / metabolism
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Animals
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology
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Bacterial Proteins / metabolism*
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Base Sequence
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Bordetella / metabolism
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Bordetella pertussis / genetics*
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Bordetella pertussis / immunology
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Bordetella pertussis / metabolism
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Bordetella pertussis / physiology
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Chloramphenicol O-Acetyltransferase / metabolism
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Chromosomes, Bacterial
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Cloning, Molecular
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DNA, Bacterial
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Female
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Mutagenesis
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Plasmids
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Sequence Analysis
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Sequence Analysis, DNA
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Trans-Activators / metabolism*
Substances
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Bacterial Proteins
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Bvg accessory factor protein, Bordetella pertussis
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DNA, Bacterial
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Recombinant Fusion Proteins
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Trans-Activators
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Chloramphenicol O-Acetyltransferase
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Alkaline Phosphatase