Abstract
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Base Sequence
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Colony Count, Microbial
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DNA, Bacterial / blood
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Francisella tularensis / genetics
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Francisella tularensis / isolation & purification*
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Humans
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Oligonucleotide Probes
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Specific Pathogen-Free Organisms
Substances
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DNA, Bacterial
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Oligonucleotide Probes