Abstract
The pathogenic human parvovirus B19 replicates only in erythroid progenitor cells. This virus was shown to bind to blood-group P antigen, as measured by hemagglutination. Erythrocytes lacking P antigen were not agglutinated with B19. Purified P antigen (globoside) blocked the binding of the virus to erythroid cells and the infectivity of the virus in a hematopoietic colony assay. Target cells were protected from infection by preincubation with monoclonal antibody to globoside. Knowledge of a parvovirus receptor has implications for understanding the pathogenesis of parvovirus infections and for the use of parvoviruses in gene therapy.
MeSH terms
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Antibodies, Monoclonal
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Capsid / metabolism
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Carbohydrate Sequence
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Cytopathogenic Effect, Viral
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Erythrocyte Membrane / immunology
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Erythrocyte Membrane / microbiology*
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Erythroid Precursor Cells / cytology
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Erythroid Precursor Cells / microbiology
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Globosides / immunology
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Globosides / metabolism*
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Globosides / pharmacology
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Hemagglutination
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Humans
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Molecular Sequence Data
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P Blood-Group System / immunology
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P Blood-Group System / metabolism*
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Parvovirus B19, Human / immunology
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Parvovirus B19, Human / metabolism*
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Parvovirus B19, Human / physiology
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Phenotype
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Receptors, Virus / metabolism*
Substances
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Antibodies, Monoclonal
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Globosides
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P Blood-Group System
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Receptors, Virus