Enhancement by microencapsulation of rotavirus-specific intestinal immune responses in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture

J Infect Dis. 1995 May;171(5):1334-8. doi: 10.1093/infdis/171.5.1334.

Abstract

The capacity of microencapsulation to enhance the humoral immune response to rotavirus in the gut-associated lymphoid tissue (GALT) of mice was determined by using a system of microencapsulation based on the ionic linkage of aqueous anionic polymers and an aqueous amine. Inoculation of mice with microencapsulated rotavirus enhanced the frequencies of virus-specific IgA-secreting cells in the lamina propria as well as the quantities of virus-specific IgA produced in GALT. In addition, an enhanced virus-specific immune response was associated with enhanced production of presumably polyclonal, non-rotavirus-specific antibodies in GALT. The mechanism by which microencapsulation enhances the humoral immune response remains to be determined.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alginates
  • Animals
  • Animals, Suckling
  • Antibodies, Viral / biosynthesis*
  • Chondroitin Sulfates
  • Culture Techniques
  • Drug Carriers
  • Drug Compounding
  • Enzyme-Linked Immunosorbent Assay / methods
  • Glucuronic Acid
  • Hexuronic Acids
  • Immunoglobulin A / biosynthesis*
  • Intestines / immunology*
  • Lymphoid Tissue / immunology*
  • Mice
  • Rotavirus / immunology*
  • Spermine
  • Vaccination

Substances

  • Alginates
  • Antibodies, Viral
  • Drug Carriers
  • Hexuronic Acids
  • Immunoglobulin A
  • Spermine
  • Glucuronic Acid
  • Chondroitin Sulfates