Rapid detection of methicillin resistance in coagulase-negative staphylococci by commercially available fluorescence test

J Clin Microbiol. 1995 Aug;33(8):2183-5. doi: 10.1128/jcm.33.8.2183-2185.1995.

Abstract

A commercially available fluorescence test (Crystal MRSA ID System; BBL) for the rapid detection of methicillin resistance in Staphylococcus aureus was evaluated for the detection of methicillin resistance in coagulase-negative staphylococci. The assay was compared with the agar dilution method and mecA detection by PCR. Provided that one uses an inoculum equivalent to a no. 2 McFarland standard instead of a no. 0.5 McFarland standard, which is used for S. aureus, the Crystal MRSA ID System detects methicillin resistance among coagulase-negative staphylococci with sensitivity, specificity, and positive and negative predictive values of 98.5, 95.4, 96.3, and 98.1%, respectively.

Publication types

  • Comparative Study

MeSH terms

  • Bacteriological Techniques* / statistics & numerical data
  • Coagulase / metabolism
  • Evaluation Studies as Topic
  • Fluorescence
  • Genes, Bacterial
  • Humans
  • Methicillin Resistance* / genetics
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / microbiology
  • Staphylococcus / drug effects*
  • Staphylococcus / enzymology
  • Staphylococcus / genetics

Substances

  • Coagulase