Inhibition of Neisseria gonorrhoeae isolates by Martin-Lewis medium. Epidemiology, susceptibility profile, and plasma analysis

Br J Vener Dis. 1982 Apr;58(2):96-100. doi: 10.1136/sti.58.2.96.

Abstract

A study was undertaken on the premise that if an increase of 1 microgram/ml in the concentration of vancomycin in Martin-Lewis medium (MLM) could improve inhibition of Gram-positive cocci, then a concomitant increase in the inhibition of gonococci could also occur. Isolates of Neisseria gonorrhoeae that failed to grow on MLM accounted for 18-5% of all positive culture results for gonorrhoea. The incidence of isolates susceptible to vancomycin was 14% and of those susceptible to trimethoprim 2.4%; one isolate was susceptible to both vancomycin and trimethoprim. The antibiotic-susceptible isolates were more frequently isolated from asymptomatic white men. Plasmid analysis showed that the 2.4-megadalton cryptic plasmic was absent in the vancomycin-susceptible isolates. The large proportion of isolates failing to grow on selective MLM has important clinical implications for the diagnosis of gonorrhea.

MeSH terms

  • Culture Media / pharmacology
  • Electrophoresis, Agar Gel
  • Female
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Neisseria gonorrhoeae / drug effects*
  • Neisseria gonorrhoeae / genetics
  • Neisseria gonorrhoeae / isolation & purification
  • Plasmids
  • Trimethoprim / pharmacology*
  • Vancomycin / pharmacology*

Substances

  • Culture Media
  • Vancomycin
  • Trimethoprim