Evaluation of the Yeast Traffic Light PNA FISH probes for identification of Candida species from positive blood cultures

Leslie Hall; Kara M Le Febre; Sharon M Deml; Sherri L Wohlfiel; Nancy L Wengenack

doi:10.1128/JCM.06148-11

Evaluation of the Yeast Traffic Light PNA FISH probes for identification of Candida species from positive blood cultures

J Clin Microbiol. 2012 Apr;50(4):1446-8. doi: 10.1128/JCM.06148-11. Epub 2012 Jan 11.

Authors

Leslie Hall¹, Kara M Le Febre, Sharon M Deml, Sherri L Wohlfiel, Nancy L Wengenack

Affiliation

¹ Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.

Abstract

The Yeast Traffic Light PNA FISH kit (YTL) correctly identified Candida spp. in 207/216 (96%) positive blood cultures. Discordant results were seen with known cross-reacting species and cultures containing Candida lambica and Rhodotorula mucilaginosa. The YTL provides rapid, reliable identification of the five common Candida species found in blood cultures.

Publication types

Evaluation Study

MeSH terms

Candida / genetics*
Candidemia / diagnosis*
Candidemia / microbiology
False Positive Reactions
Fluorescent Dyes / chemistry*
Humans
In Situ Hybridization, Fluorescence* / methods
In Situ Hybridization, Fluorescence* / standards
Peptide Nucleic Acids / chemistry*
Reference Standards
Sensitivity and Specificity

Substances

Fluorescent Dyes
Peptide Nucleic Acids