Characterization of CIA-1, an Ambler class A extended-spectrum β-lactamase from Chryseobacterium indologenes

Antimicrob Agents Chemother. 2012 Jan;56(1):588-90. doi: 10.1128/AAC.05165-11. Epub 2011 Nov 14.

Abstract

An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B, C. indologenes has a class A ESBL gene, bla(CIA-1), located on the chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / administration & dosage*
  • Chromosomes, Bacterial / genetics
  • Chryseobacterium / drug effects
  • Chryseobacterium / genetics*
  • Chryseobacterium / isolation & purification
  • Cloning, Molecular
  • Escherichia coli
  • Flavobacteriaceae Infections / drug therapy*
  • Flavobacteriaceae Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Recombinant Proteins / classification
  • Recombinant Proteins / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • beta-Lactam Resistance*
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Recombinant Proteins
  • beta-Lactamases