Abstract
An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B, C. indologenes has a class A ESBL gene, bla(CIA-1), located on the chromosome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / administration & dosage*
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Chromosomes, Bacterial / genetics
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Chryseobacterium / drug effects
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Chryseobacterium / genetics*
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Chryseobacterium / isolation & purification
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Cloning, Molecular
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Escherichia coli
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Flavobacteriaceae Infections / drug therapy*
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Flavobacteriaceae Infections / microbiology
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Recombinant Proteins / classification
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Recombinant Proteins / genetics
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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beta-Lactam Resistance*
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beta-Lactamases / classification
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Recombinant Proteins
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beta-Lactamases