Immunodominant T-cell responses to dengue virus NS3 are associated with DHF

Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16922-7. doi: 10.1073/pnas.1010867107. Epub 2010 Sep 13.

Abstract

Dengue infections are increasing at an alarming rate in many tropical and subtropical countries, where epidemics can put health care systems under extreme pressure. The more severe infections lead to dengue hemorrhagic fever (DHF), which can be life threatening. A variety of viral and host factors have been associated with the severity of dengue infections. Because secondary dengue infection is more commonly associated with DHF than primary infections, the acquired immune response to dengue, both B cells and T cells have been implicated. In this study, we set out to study T-cell responses across the entire dengue virus proteome and to see whether these were related to disease severity in a cohort of dengue-infected children from Thailand. Robust responses were observed in most infected individuals against most viral proteins. Responses to NS3 were the most frequent, and there was a very strong association between the magnitude of the response and disease severity. Furthermore, in DHF, cytokine-high CD107a-negative cells predominated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dengue Virus / enzymology
  • Dengue Virus / immunology*
  • HLA-A Antigens / immunology
  • Humans
  • Proteome
  • Serine Endopeptidases / immunology*
  • Severe Dengue / immunology*
  • T-Lymphocytes / immunology*

Substances

  • HLA-A Antigens
  • Proteome
  • NS3 protease, dengue virus
  • Serine Endopeptidases