Evaluation of the integrated database network system (IDNS) SmartGene software for analysis of 16S rRNA gene sequences for identification of Nocardia species

J Clin Microbiol. 2010 Aug;48(8):2995-8. doi: 10.1128/JCM.00681-10. Epub 2010 Jun 23.

Abstract

16S rRNA gene sequences of 102 Nocardia isolates were analyzed using the Integrated Database Network System (IDNS) SmartGene centroid database. A total of 76% of the isolates were correctly identified. Discordant identifications were due to inadequate centroid length (3 species), inaccurate or insufficient entries in the public databases (5 species), and heterogeneous sequences among members of a species (1 species).

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • DNA, Bacterial / genetics*
  • Databases, Genetic*
  • Diagnostic Errors / statistics & numerical data
  • Humans
  • Nocardia / classification
  • Nocardia / genetics*
  • Nocardia / isolation & purification*
  • Nocardia Infections / diagnosis*
  • Nocardia Infections / microbiology
  • RNA, Ribosomal, 16S / genetics*
  • Software

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S