Nosocomial infections are important problem for the modern medicine. Especially sensitive category, susceptible to nosocomial infections, are the infants. The aim of the research was to establish an active monitoring over the breaking out of the nosocomial infections in the Neonatology Department of the Gynecology and Obstetrics Clinic of the University Clinical Center Tuzla (UKC) and to determine the kind of bacteria that causes the nosocomial infections as well as the type of infection according to anatomical localization. The research encompassed 8000 of newborn infants at the Neonatology Department of the UKC Tuzla in the years 2005 and 2006. The determining of the nosocomial infections was done by using the standard CDC criteria. The results show that in two researching years the leading nosocomial infections were bacteriemia, followed by other infections, respiratory tract infections and urinary tract infections. The mostly identified carrier of the nosocomial infections in 2005 were gram-positive bacteria (65,5%): coagulase-negative staphylococci was isolated in 54,6%, Staphylococcus aureus in 5,5% and Streptococcus species in 5,5% of cases (p<0,001). The isolated gram-negative bacteria, carriers of nosocomial infections in 2005 were Klebisiella pneumoniae (21,8%), Escherichia coli (5,5%), Pseudomanas aeruginosa (3,6%) and Morganella morganii (3,6%) (p<0,05). In 2006 the mostly identified and isolated carriers of nosocomial infections were gram positive bacteria (64,2%): coagulase-negative Staphylococci (43,3%), Streptococcus species (13,4%) and Staphylococcus aureus (7,5%) (p<0,001). Gram negative bacteria, carriers of nosocomial infections in 2006 were Klebsiella pneumoniae (19,4%), Escherichia coli (10,5%), Acinetobacter species (3%), Pseudomonas aeruginosa (1,5%) and Proteus mirabilis (1,5%) (p<0,05). The bacteriemia was mostly caused by coagulase-negative staphylococcus. Infections of the urinary tract were caused by gram-negative bacteria Klebsiella pneumoniae and Escherichia coli. Other infections were caused by coagulase-negative Staphylococci, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli. Infections of the respiratory tract were caused by Streptococcus species, Klebsiella pneumoniae, coagulase-negative Staphylococci, Staphylococcus aureus and Pseudomonas aeruginosa. The most frequent carriers of the nosocomial infections at the Neonatology Department of the UKC Tuzla in 2005 and 2006 was gram-positive bacteria-coagulase-negative Staphylococci.