Production and characterization of a human hyperimmune intravenous immunoglobulin against Pseudomonas aeruginosa and Klebsiella species

J Infect Dis. 1991 May;163(5):1055-61. doi: 10.1093/infdis/163.5.1055.

Abstract

A hyperimmune globulin for intravenous use (H-IVIG) was prepared from the plasma of donors immunized with Pseudomonas aeruginosa and Klebsiella vaccines. H-IVIG preparations contained substantially higher IgG antibody levels to all nine P. aeruginosa vaccine antigens and to 22 of the 24 Klebsiella vaccine antigens than did commercial IVIG. The H-IVIG was more effective at promoting the opsonophagocytic killing of P. aeruginosa and Klebsiella vaccine serotype strains than normal IVIG. The H-IVIG neutralized greater than 20 times more toxin A than commercial IVIG. Only the H-IVIG offered significant protection against Klebsiella K2 sepsis. The H-IVIG provided significantly better protection against six of the eight P. aeruginosa vaccine serotypes than normal IVIG when compared in a murine burn wound sepsis model. The H-IVIG also protected mice against an Enterobacter aerogenes challenge, whereas normal IVIG was ineffective.

MeSH terms

  • Animals
  • Bacterial Vaccines / immunology*
  • Enterobacter
  • Enterobacteriaceae Infections / prevention & control
  • Humans
  • Immune Sera / immunology
  • Immunization, Passive*
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification
  • Immunoglobulins / immunology*
  • Immunoglobulins / isolation & purification
  • Immunoglobulins, Intravenous
  • Klebsiella / immunology*
  • Klebsiella Infections / prevention & control
  • Mice
  • Opsonin Proteins / immunology
  • Phagocytosis
  • Pseudomonas Infections / prevention & control
  • Pseudomonas aeruginosa / immunology*

Substances

  • Bacterial Vaccines
  • Immune Sera
  • Immunoglobulin G
  • Immunoglobulins
  • Immunoglobulins, Intravenous
  • Opsonin Proteins