TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food

Appl Environ Microbiol. 2008 Oct;74(20):6465-9. doi: 10.1128/AEM.01459-08. Epub 2008 Aug 29.

Abstract

A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.

Publication types

  • Evaluation Study

MeSH terms

  • Base Sequence
  • DNA Primers / genetics
  • Food Microbiology*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / standards
  • Reference Standards
  • Sensitivity and Specificity
  • Yersinia pseudotuberculosis / genetics
  • Yersinia pseudotuberculosis / isolation & purification*

Substances

  • DNA Primers