Abstract
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.
MeSH terms
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Base Sequence
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DNA Primers / genetics
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Food Microbiology*
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Genes, Bacterial
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / standards
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Reference Standards
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Sensitivity and Specificity
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Yersinia pseudotuberculosis / genetics
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Yersinia pseudotuberculosis / isolation & purification*