Abstract
We examined 73 children with respiratory infections for Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae using real-time PCR assay and serological tests. C. pneumoniae and M. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. The sensitivities and specificities of real-time PCR versus definite diagnosis of acute infection were 63.6% and 100% for C. pneumoniae, and 100% and 100% for M. pneumoniae, respectively. C. pneumoniae PCR-negative results appeared to be due to poor growth of the organism. The sensitivity and specificity of ImmunoCard tests were 33.3% and 82.1%, respectively, indicating that the efficacy of rapid diagnosis was disputable. The present results suggest that real-time PCR is suitable for rapid diagnosis as a first screening test to determine first-line antibacterial agents to be used against these infectious diseases.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adolescent
-
Agglutination Tests
-
Antibodies, Bacterial / blood
-
Antigens, Bacterial / immunology
-
Child
-
Child, Preschool
-
Chlamydophila Infections / diagnosis*
-
Chlamydophila Infections / epidemiology
-
Chlamydophila Infections / immunology
-
Chlamydophila Infections / microbiology
-
Chlamydophila pneumoniae / immunology
-
Chlamydophila pneumoniae / isolation & purification
-
Enzyme-Linked Immunosorbent Assay
-
Female
-
Humans
-
Infant
-
Japan / epidemiology
-
Male
-
Mycoplasma pneumoniae / immunology
-
Mycoplasma pneumoniae / isolation & purification
-
Pneumonia, Mycoplasma / diagnosis*
-
Pneumonia, Mycoplasma / epidemiology
-
Pneumonia, Mycoplasma / immunology
-
Pneumonia, Mycoplasma / microbiology
-
Polymerase Chain Reaction*
-
Prospective Studies
-
Respiratory Tract Infections / diagnosis*
-
Respiratory Tract Infections / epidemiology
-
Respiratory Tract Infections / immunology
-
Respiratory Tract Infections / microbiology
-
Sensitivity and Specificity
Substances
-
Antibodies, Bacterial
-
Antigens, Bacterial