Abstract
Nucleotide variation in the Pneumocystis jirovecii upstream conserved sequence (UCS) and kexin-like serine protease (Kex1) loci was studied in pulmonary specimens from Portuguese HIV-positive patients. DNA was extracted and used for specific molecular sequence analysis. The number of UCS tandem repeats detected in 13 successfully sequenced isolates ranged from three (9 isolates, 69%) to four (4 isolates, 31%). A novel tandem repeat pattern and two novel polymorphisms were detected in the UCS region. For the Kex1 gene, the wild-type (24 isolates, 86%) was the most frequent sequence detected among the 28 sequenced isolates. Nevertheless, a nonsynonymous (1 isolate, 3%) and three synonymous (3 isolates, 11%) polymorphisms were detected and are described here for the first time.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bronchoalveolar Lavage Fluid / microbiology
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Conserved Sequence / genetics*
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DNA, Fungal / analysis*
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Fungal Proteins / genetics
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HIV Seropositivity / complications
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Humans
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Molecular Sequence Data
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Pneumocystis carinii / genetics*
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Pneumonia, Pneumocystis / complications
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Pneumonia, Pneumocystis / microbiology*
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Polymerase Chain Reaction
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Polymorphism, Genetic
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Portugal
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Sequence Alignment
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Sequence Analysis, DNA
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Serine Endopeptidases / genetics*
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Sputum / microbiology
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Tandem Repeat Sequences
Substances
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DNA, Fungal
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Fungal Proteins
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Kex1 protein, Pneumocystis jirovecii
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Serine Endopeptidases
Associated data
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GENBANK/EU431323
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GENBANK/EU431324
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GENBANK/EU431325
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GENBANK/EU431326
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GENBANK/EU431327
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GENBANK/EU437385
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GENBANK/EU797518
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GENBANK/EU797519