Real-time PCR assay to detect DNA in sera for the diagnosis of deep-seated trichosporonosis

Microbiol Immunol. 2007;51(6):633-5. doi: 10.1111/j.1348-0421.2007.tb03950.x.

Abstract

Deep-seated trichosporonosis due to Trichosporon asahii is life-threatening and has high mortality. A real-time PCR assay to detect T. asahii DNA in sera for diagnosis of this fungal infection was developed. The assay showed a higher sensitivity than polysaccharide antigen detection method. Our new real-time PCR assay may be used for diagnosing deep-seated trichosporonosis due to T. asahii.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Fungal / blood*
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Fungemia / blood*
  • Fungemia / microbiology
  • Humans
  • Polymerase Chain Reaction / methods*
  • Trichosporon / genetics*
  • Trichosporon / isolation & purification

Substances

  • DNA, Fungal
  • DNA, Ribosomal Spacer