Histoplasmosis-associated cross-reactivity in the BioRad Platelia Aspergillus enzyme immunoassay

Clin Vaccine Immunol. 2007 May;14(5):638-40. doi: 10.1128/CVI.00479-06. Epub 2007 Mar 7.

Abstract

We observed false-positive results in the Platelia Aspergillus enzyme-linked immunoassay (EIA) for specimens from patients with histoplasmosis and mice with experimental infection. Platelia Aspergillus EIA-positive specimens were negative in the second-generation Histoplasma antigen EIA. Care must be taken to exclude histoplasmosis for patients with positive Platelia Aspergillus EIA results.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Antibodies, Fungal*
  • Antigens, Fungal / blood*
  • Aspergillosis, Allergic Bronchopulmonary / diagnosis
  • Cross Reactions
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Galactose / analogs & derivatives
  • Histoplasmosis / blood*
  • Histoplasmosis / immunology*
  • Humans
  • Mannans / immunology
  • Mice
  • Rabbits

Substances

  • Antibodies, Fungal
  • Antigens, Fungal
  • Mannans
  • galactomannan
  • Galactose