Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

J Virol. 1992 Feb;66(2):1273-6. doi: 10.1128/JVI.66.2.1273-1276.1992.

Abstract

Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

MeSH terms

  • Blood
  • Blotting, Southern
  • Cells, Cultured
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Enzyme-Linked Immunosorbent Assay
  • Hematopoietic Stem Cells / physiology*
  • Humans
  • Kinetics
  • Molecular Weight
  • Parvovirus B19, Human / genetics
  • Parvovirus B19, Human / isolation & purification
  • Parvovirus B19, Human / physiology*
  • Viral Proteins / analysis
  • Virus Replication*

Substances

  • DNA, Viral
  • Viral Proteins