Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene

Eur J Clin Microbiol Infect Dis. 2007 Feb;26(2):131-5. doi: 10.1007/s10096-007-0254-z.

Abstract

In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Bacterial Toxins / genetics*
  • Bacterial Toxins / metabolism
  • Culture Media
  • Exotoxins / genetics*
  • Exotoxins / metabolism
  • Humans
  • Leukocidins / genetics*
  • Leukocidins / metabolism
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / isolation & purification*
  • Time Factors

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Culture Media
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • leukocidin S-component protein, Staphylococcus