Abstract
In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Bacterial Toxins / genetics*
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Bacterial Toxins / metabolism
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Culture Media
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Exotoxins / genetics*
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Exotoxins / metabolism
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Humans
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Leukocidins / genetics*
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Leukocidins / metabolism
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Staphylococcus aureus / genetics
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Staphylococcus aureus / growth & development
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Staphylococcus aureus / isolation & purification*
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Time Factors
Substances
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Bacterial Proteins
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Bacterial Toxins
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Culture Media
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Exotoxins
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Leukocidins
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Panton-Valentine leukocidin
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leukocidin S-component protein, Staphylococcus