Direct identification of Mycobacterium haemophilum in skin lesions of immunocompromised patients by PCR-restriction endonuclease analysis

S X Wang; L H Sng; H N Leong; B H Tan

doi:10.1128/JCM.42.7.3336-3338.2004

Direct identification of Mycobacterium haemophilum in skin lesions of immunocompromised patients by PCR-restriction endonuclease analysis

J Clin Microbiol. 2004 Jul;42(7):3336-8. doi: 10.1128/JCM.42.7.3336-3338.2004.

Authors

S X Wang¹, L H Sng, H N Leong, B H Tan

Affiliation

¹ Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608. gptwsx@sgh.com.sg

Abstract

PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 weeks required for culture isolation and identification by conventional methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Restriction Enzymes / pharmacology
Humans
Immunocompromised Host
Mycobacterium haemophilum / isolation & purification*
Polymerase Chain Reaction / methods*
Skin / injuries
Skin / microbiology*

Substances

DNA Restriction Enzymes