Abstract
We tested a real-time PCR assay targeting the Pneumocystis jirovecii mitochondrial large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non-immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested-PCR assay targeting the same locus. Because of its one-step procedure, and its sensitivity and rapidity, the real-time PCR assay is particularly suitable for screening individuals parasitized by P. jirovecii within large populations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Bronchiolitis / immunology
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Bronchiolitis / microbiology
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DNA Primers
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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DNA, Mitochondrial / genetics*
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DNA, Mitochondrial / isolation & purification
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Disease Reservoirs
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Gene Amplification
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Genes, Reporter
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Humans
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Immunocompetence / immunology*
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Infant
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Pneumocystis carinii / genetics*
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Pneumocystis carinii / immunology*
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Pneumonia, Pneumocystis / immunology*
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Polymerase Chain Reaction / methods
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RNA, Fungal / genetics
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RNA, Ribosomal / genetics*
Substances
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DNA Primers
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DNA, Fungal
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DNA, Mitochondrial
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RNA, Fungal
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RNA, Ribosomal