Abstract
Spontaneous polymyxin-resistant mutants of Pseudomonas aeruginosa were isolated. The mutations responsible for this phenotype were mapped to a two-component signal transduction system similar to PmrAB of Salmonella enterica serovar Typhimurium. Lipid A of these mutants contained aminoarabinose, an inducible modification that is associated with polymyxin resistance. Thus, P. aeruginosa possesses a mechanism that induces resistance to cationic antimicrobial peptides in response to environmental conditions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antimicrobial Cationic Peptides
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Arabinose / analogs & derivatives*
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Arabinose / chemistry*
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Bacterial Proteins*
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Base Sequence
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Chromosome Mapping
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Drug Resistance, Bacterial
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Gene Expression Regulation, Bacterial
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Lipid A / chemistry*
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Molecular Sequence Data
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Mutation
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Peptides / pharmacology*
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Polymyxin B / pharmacology*
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Proteins / pharmacology*
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / genetics
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Transcription Factors / genetics
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Transcription Factors / physiology*
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beta-Defensins / pharmacology*
Substances
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Antimicrobial Cationic Peptides
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Bacterial Proteins
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C18G peptide
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DEFB4A protein, human
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Lipid A
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Peptides
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PmrB protein, bacteria
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Proteins
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Transcription Factors
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aminoarabinose
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beta-Defensins
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protegrin-1
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Arabinose
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Polymyxin B
Associated data
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GENBANK/AY493419
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GENBANK/AY493420
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GENBANK/AY493421