Use of high-performance liquid chromatography to study the pharmacokinetics of colistin sulfate in rats following intravenous administration

Antimicrob Agents Chemother. 2003 May;47(5):1766-70. doi: 10.1128/AAC.47.5.1766-1770.2003.

Abstract

The pharmacokinetics of colistin was investigated using specific high-performance liquid chromatography (HPLC) to measure the concentrations of colistin and colistin A and B in plasma and urine in five rats after administration of an intravenous bolus of 1 mg of colistin sulfate/kg of body weight. There were differences in the pharmacokinetic behaviors of unbound colistin A and B. This is the first report of the use of HPLC to study the pharmacokinetics of colistin and its two major components.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacokinetics*
  • Chromatography, High Pressure Liquid
  • Colistin / administration & dosage
  • Colistin / analysis
  • Colistin / pharmacokinetics*
  • Injections, Intravenous
  • Male
  • Metabolic Clearance Rate
  • Protein Binding
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Anti-Bacterial Agents
  • Colistin