Abstract
The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta-lactamases were enlarged by </=1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins*
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Cefpodoxime
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Ceftazidime / pharmacology
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Ceftizoxime / analogs & derivatives*
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Ceftizoxime / pharmacology*
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Cephalosporins / pharmacology*
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Clavulanic Acid / pharmacology
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Humans
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Klebsiella / drug effects
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Klebsiella / enzymology*
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Klebsiella Infections / microbiology*
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / enzymology*
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Microbial Sensitivity Tests / methods
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beta-Lactamases / metabolism*
Substances
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Bacterial Proteins
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Cephalosporins
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Clavulanic Acid
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Ceftazidime
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Ceftizoxime
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AmpC beta-lactamases
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beta-Lactamases