Abstract
A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Fungal / genetics
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DNA Primers
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DNA, Fungal / analysis
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Fungal Proteins / genetics*
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Glycoproteins*
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Humans
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Paracoccidioides / genetics
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Paracoccidioides / isolation & purification*
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Paracoccidioidomycosis / diagnosis*
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Paracoccidioidomycosis / microbiology
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Polymerase Chain Reaction / methods*
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Sputum / microbiology*
Substances
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43 kDa protein, Paracoccidioides
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Antigens, Fungal
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DNA Primers
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DNA, Fungal
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Fungal Proteins
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Glycoproteins