Abstract
A new natural TEM-2 derivative, named TEM-72, was identified in a Proteus mirabilis strain and in a Morganella morganii strain isolated in Italy in 1999. Compared to TEM-1, TEM-72 contains the following amino acid substitutions: Q39K, M182T, G238S, and E240K. Kinetic analysis showed that TEM-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. Expression of bla(TEM-72) in Escherichia coli was capable of decreasing the host susceptibility to the above drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology
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DNA, Bacterial / analysis
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Humans
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Hydrolysis
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Italy
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Kinetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Morganella morganii / drug effects
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Morganella morganii / enzymology*
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Morganella morganii / genetics
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Morganella morganii / isolation & purification
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Proteus mirabilis / drug effects
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Proteus mirabilis / enzymology*
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Proteus mirabilis / genetics
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Proteus mirabilis / isolation & purification
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beta-Lactamases / analysis*
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beta-Lactamases / genetics
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beta-Lactamases / isolation & purification
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beta-Lactamases / metabolism
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beta-Lactams
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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beta-Lactams
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beta-lactamase TEM-72
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beta-Lactamases
Associated data
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GENBANK/AF157413
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GENBANK/AF157553