Molecular epidemiology of an outbreak due to IRT-2 beta-lactamase-producing strains of Klebsiella pneumoniae in a geriatric department

J Antimicrob Chemother. 2000 Apr;45(4):467-73. doi: 10.1093/jac/45.4.467.

Abstract

In February 1998, 195 patients in the geriatric department of a French hospital were screened for the presence of co-amoxiclav-resistant Klebsiella pneumoniae. Eleven co-amoxiclav-resistant isolates obtained all produced an identical IRT-2 beta-lactamase. These K. pneumoniae isolates were clonally related and harboured a c. 55 kb non-conjugative plasmid encoding a non-class-1 integron-located blaIRT-2 gene. This study underlines that geriatric departments may be a reservoir for antibiotic-resistant strains and that IRT beta-lactamase-producing strains may be nosocomial pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amoxicillin-Potassium Clavulanate Combination / pharmacology
  • Bacterial Proteins*
  • Cross Infection / microbiology*
  • DNA, Bacterial / genetics
  • Drug Resistance, Microbial
  • Electroporation
  • France
  • Geriatrics
  • Hospital Departments
  • In Situ Hybridization
  • Isoelectric Focusing
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / metabolism
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Phenotype
  • Reverse Transcriptase Polymerase Chain Reaction
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Amoxicillin-Potassium Clavulanate Combination
  • TEM24 beta-lactamase
  • beta-lactamase IRT-2
  • beta-Lactamases