Choline uptake in the end-plate rich area of rat diaphragm is saturable between 0.7 and 16 micron. This choline uptake obeys hyperbolic kinetics and indicates the presence of high-affinity carrier with Km values of 11.8 and 13.2 micron and Vmax values of 37 and 31 nmol choline/h/g tissue respectively. In the presence of hemicholinium-3 the carrier shows sigmoidal kinetics, and hemicholinium-3 is apparently a cooperative effector of choline uptake. The carrier is more calcium than sodium dependent.