Abstract
Randomly amplified polymorphic DNA analysis and rRNA gene restriction patterns (ribotyping) were compared as methods of investigating a nosocomial outbreak of nocardiosis involving three heart transplant recipients. No clear distinctions between three clinically related isolates and four unrelated strains were obtained by ribotyping. On the contrary, randomly amplified polymorphic DNA analysis with two selected primers, primers 2650 and DKU49, showed one pattern for the three related isolates and four patterns for the unrelated strains.
Publication types
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Case Reports
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Comparative Study
MeSH terms
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Adult
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Base Sequence
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Cross Infection / epidemiology*
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Cross Infection / etiology
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Cross Infection / microbiology
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DNA Primers / genetics
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DNA Restriction Enzymes
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Disease Outbreaks*
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Evaluation Studies as Topic
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France / epidemiology
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Heart Transplantation / adverse effects*
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Nocardia / genetics
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Nocardia / isolation & purification
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Nocardia Infections / epidemiology*
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Nocardia Infections / etiology
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Nocardia Infections / microbiology
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RNA, Ribosomal / genetics
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Random Amplified Polymorphic DNA Technique*
Substances
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DNA Primers
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RNA, Ribosomal
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DNA Restriction Enzymes