Abstract
Two clinical strains of Klebsiella pneumoniae, TP 01 and TP 02, presented resistance to amoxicillin-clavulanate and were fully susceptible to cephalothin. These strains produced two beta-lactamases, SHV-1 and a TEM enzyme with a pI of 5.2. The previously described changes Arg-244-->Cys and Arg-244-->Ser in IRT-1 and IRT-2 (A. Belaaouaj, C. Lapoumeroulie, M. M. Caniça, G. Vedel, P. Nevot, R. Krishnamoorthy, and G. Paul, FEMS Microbiol. Lett. 120:75-80, 1994) were found in TEM enzymes from the TP 01 and TP 02 strains, respectively. This is the first report of inhibitor-resistant TEM (IRT) in species other than Escherichia coli from the family Enterobacteriaceae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amoxicillin / pharmacology
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Amoxicillin-Potassium Clavulanate Combination
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Anti-Bacterial Agents / pharmacology*
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Cephalosporins / pharmacology
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Cephalothin / pharmacology
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Clavulanic Acids / pharmacology
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DNA, Bacterial / analysis
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Drug Resistance, Microbial
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Drug Therapy, Combination / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Enzyme Inhibitors / pharmacology*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Isoelectric Focusing
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Klebsiella pneumoniae / drug effects*
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics
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Microbial Sensitivity Tests
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Polymerase Chain Reaction
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beta-Lactamase Inhibitors*
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beta-Lactamases / chemistry
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Cephalosporins
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Clavulanic Acids
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DNA, Bacterial
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Enzyme Inhibitors
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beta-Lactamase Inhibitors
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Amoxicillin-Potassium Clavulanate Combination
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Amoxicillin
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beta-lactamase PIT-2
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beta-Lactamases
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Cephalothin