Abstract
A battery of morphological, physiological, and biochemical tests, including paper chromatographic analysis of whole cell hydrolysates, was used to study 177 cultures of aerobic actinomycetes. One hundred thirty-five of the 177 were submitted as diagnostic laboratory specimens; of these, 129 were identified as belonging to 1 of 10 genus or species groups. The tests and procedures used were found to be relatively easy to perform and interpret. On the basis of the results, a flow chart was devised to permit the step by step identification of unknown clinical isolates of these organisms.
MeSH terms
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Acids / biosynthesis
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Actinomycetales / analysis
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Actinomycetales / classification*
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Actinomycetales / cytology
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Actinomycetales / drug effects
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Actinomycetales / growth & development
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Actinomycetales / metabolism
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Aerobiosis
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Bacteriological Techniques*
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Caseins / metabolism
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Chromatography, Paper
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Drug Resistance, Microbial
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Flavonoids / metabolism
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Hippurates / metabolism
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Hypoxanthines / metabolism
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Monosaccharides / analysis
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Muramidase / pharmacology
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Pimelic Acids / analysis
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Temperature
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Terminology as Topic
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Tyrosine / metabolism
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Urea / metabolism
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Xanthines / metabolism
Substances
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Acids
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Caseins
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Flavonoids
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Hippurates
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Hypoxanthines
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Monosaccharides
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Pimelic Acids
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Xanthines
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Tyrosine
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Urea
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Muramidase