Laboratory identification of clinically important aerobic actinomycetes

Appl Microbiol. 1973 Apr;25(4):665-81. doi: 10.1128/am.25.4.665-681.1973.

Abstract

A battery of morphological, physiological, and biochemical tests, including paper chromatographic analysis of whole cell hydrolysates, was used to study 177 cultures of aerobic actinomycetes. One hundred thirty-five of the 177 were submitted as diagnostic laboratory specimens; of these, 129 were identified as belonging to 1 of 10 genus or species groups. The tests and procedures used were found to be relatively easy to perform and interpret. On the basis of the results, a flow chart was devised to permit the step by step identification of unknown clinical isolates of these organisms.

MeSH terms

  • Acids / biosynthesis
  • Actinomycetales / analysis
  • Actinomycetales / classification*
  • Actinomycetales / cytology
  • Actinomycetales / drug effects
  • Actinomycetales / growth & development
  • Actinomycetales / metabolism
  • Aerobiosis
  • Bacteriological Techniques*
  • Caseins / metabolism
  • Chromatography, Paper
  • Drug Resistance, Microbial
  • Flavonoids / metabolism
  • Hippurates / metabolism
  • Hypoxanthines / metabolism
  • Monosaccharides / analysis
  • Muramidase / pharmacology
  • Pimelic Acids / analysis
  • Temperature
  • Terminology as Topic
  • Tyrosine / metabolism
  • Urea / metabolism
  • Xanthines / metabolism

Substances

  • Acids
  • Caseins
  • Flavonoids
  • Hippurates
  • Hypoxanthines
  • Monosaccharides
  • Pimelic Acids
  • Xanthines
  • Tyrosine
  • Urea
  • Muramidase