Rapid detection of Campylobacter antigen by enzyme immunoassay leads to increased positivity rates

Carmen L Giltner; Sandra Saeki; April M Bobenchik; Romney M Humphries

doi:10.1128/JCM.02565-12

Rapid detection of Campylobacter antigen by enzyme immunoassay leads to increased positivity rates

J Clin Microbiol. 2013 Feb;51(2):618-20. doi: 10.1128/JCM.02565-12. Epub 2012 Nov 21.

Authors

Carmen L Giltner¹, Sandra Saeki, April M Bobenchik, Romney M Humphries

Affiliation

¹ David Geffen School of Medicine, Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA.

Abstract

Campylobacter antigen detection by enzyme immunoassay (EIA) provides rapid results compared to traditional culture. However, concern exists regarding specificity. Verification studies of an EIA compared to culture revealed a positive predictive value (PPV) of 91%, whereas PPV fell to 42% during routine diagnostic testing. We suggest all positive EIA results be confirmed via culture.

MeSH terms

Antigens, Bacterial / immunology*
Campylobacter / immunology*
Campylobacter / isolation & purification
Campylobacter Infections / diagnosis*
Humans
Immunoenzyme Techniques* / methods
Sensitivity and Specificity

Substances

Antigens, Bacterial