Abstract
A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Cholera / microbiology
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Cholera Toxin / genetics
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DNA-Binding Proteins / genetics
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Electrophoresis, Agar Gel
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Fimbriae Proteins / genetics
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Humans
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Transcription Factors / genetics
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Vibrio cholerae O1 / classification*
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Vibrio cholerae O1 / genetics*
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Vibrio cholerae O139 / classification*
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Vibrio cholerae O139 / genetics*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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TcpA protein, Vibrio cholerae
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Transcription Factors
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toxR protein, Vibrio cholerae
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Fimbriae Proteins
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Cholera Toxin