Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow

J Clin Microbiol. 2008 Apr;46(4):1537-40. doi: 10.1128/JCM.00030-08. Epub 2008 Feb 20.

Abstract

We developed the simple, rapid (1 h), and accurate PNA FISH(Flow) method for the identification of Candida albicans. The method exploits unique in solution in situ hybridization conditions under which the cells are simultaneously fixed and hybridized. This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy.

Publication types

  • Evaluation Study

MeSH terms

  • Candida albicans / classification*
  • Candida albicans / genetics
  • Candida albicans / isolation & purification
  • Candidiasis / microbiology*
  • Flow Cytometry
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Microscopy, Fluorescence
  • Nucleic Acid Probes / genetics*
  • Peptide Nucleic Acids*
  • Reagent Kits, Diagnostic*
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Nucleic Acid Probes
  • Peptide Nucleic Acids
  • Reagent Kits, Diagnostic