Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp

Ron Boot; Lia Van de Berg; Frans A G Reubsaet; Maurice J Vlemminx

doi:10.1016/j.vetmic.2007.10.007

Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp

Vet Microbiol. 2008 Apr 30;128(3-4):395-9. doi: 10.1016/j.vetmic.2007.10.007. Epub 2007 Oct 16.

Authors

Ron Boot¹, Lia Van de Berg, Frans A G Reubsaet, Maurice J Vlemminx

Affiliation

¹ Section of Laboratory Animal Microbiology, Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. r.boot@rivm.nl

PMID: 18023543
DOI: 10.1016/j.vetmic.2007.10.007

Abstract

Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.

MeSH terms

Animals
Base Sequence
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
False Positive Reactions
Fusobacterium Infections / diagnosis
Fusobacterium Infections / microbiology
Fusobacterium Infections / veterinary*
Guinea Pigs / microbiology*
Leptotrichia / isolation & purification*
Molecular Sequence Data
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / standards
Polymerase Chain Reaction / veterinary*
Rodent Diseases / diagnosis*
Rodent Diseases / microbiology
Sequence Alignment / veterinary
Species Specificity
Streptobacillus / isolation & purification*

Substances

DNA, Bacterial
DNA, Ribosomal