Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species

J Clin Microbiol. 2007 Nov;45(11):3802-3. doi: 10.1128/JCM.01127-07. Epub 2007 Sep 5.

Abstract

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

MeSH terms

  • Candida / isolation & purification*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Nucleic Acid Probes / genetics*
  • Peptide Nucleic Acids / genetics*

Substances

  • Nucleic Acid Probes
  • Peptide Nucleic Acids