Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

J Med Microbiol. 2005 Sep;54(Pt 9):815-821. doi: 10.1099/jmm.0.46042-0.

Abstract

The role of purified iron-regulated outer-membrane proteins (IROMPs) from Salmonella enterica serotype Typhi in modulation of specific T-cell responses was studied. The cellular immune response induced by IROMPs was measured by assessing the delayed-type hypersensitivity (DTH) response, lymphocyte proliferation, T-cell phenotyping and cytokine-producing cells using lymphocytes isolated from the spleen and Peyer's patches of IROMPs-immunized, immunized-challenged, infected and control mice. IROMPs immunization resulted in an enhanced DTH response and exhibited a significant increase in the protein-specific proliferative response of lymphocyte from the spleen as well as Peyer's patches. A significant increase was also observed in the ratio of CD4+/CD8+ cells in the immunized mice as compared to the infected mice. Results of the cytokine analysis revealed that during the initial period there was increased production of interleukin (IL)-2- and interferon (IFN)-gamma-producing cells in the spleen and Peyer's patches, indicating a Th1 type response, whereas in the later period of the study, increased production of IL-4-producing cells suggested a Th2 type response. The results of this study suggest a role for S. Typhi IROMPs in modulating the cellular immune response at peripheral and mucosal levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Outer Membrane Proteins / administration & dosage
  • Bacterial Outer Membrane Proteins / immunology*
  • Cytokines
  • Female
  • Hypersensitivity, Delayed
  • Immunization
  • Immunophenotyping
  • Iron / metabolism*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology
  • Salmonella typhi / immunology*
  • Salmonella typhi / metabolism
  • Spleen / cytology
  • Spleen / immunology
  • T-Lymphocytes / immunology*

Substances

  • Bacterial Outer Membrane Proteins
  • Cytokines
  • Iron