Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures

J Clin Microbiol. 2005 Jun;43(6):2909-12. doi: 10.1128/JCM.43.6.2909-2912.2005.

Abstract

We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.

Publication types

  • Evaluation Study
  • Multicenter Study

MeSH terms

  • Blood / microbiology
  • Candida albicans / classification*
  • Candida albicans / genetics
  • Candida albicans / isolation & purification
  • Candidiasis / microbiology
  • Culture Media
  • Fungemia / microbiology*
  • Humans
  • In Situ Hybridization, Fluorescence* / methods
  • Nucleic Acid Probes / genetics*
  • Peptide Nucleic Acids / genetics*
  • Predictive Value of Tests
  • Sensitivity and Specificity

Substances

  • Culture Media
  • Nucleic Acid Probes
  • Peptide Nucleic Acids