Evaluation of nested PCR in diagnosis of typhoid fever

J Clin Microbiol. 2005 Jan;43(1):431-2. doi: 10.1128/JCM.43.1.431-432.2005.

Abstract

In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests
  • Blood / microbiology
  • Child
  • Child, Preschool
  • Culture Media
  • Humans
  • Infant
  • Polymerase Chain Reaction / methods*
  • Salmonella typhi / genetics
  • Salmonella typhi / isolation & purification*
  • Sensitivity and Specificity
  • Typhoid Fever / diagnosis*

Substances

  • Culture Media