Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates

Banu Sancak; John H Rex; Enuo Chen; Kieren Marr

doi:10.1128/JCM.42.12.5889-5891.2004

Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates

J Clin Microbiol. 2004 Dec;42(12):5889-91. doi: 10.1128/JCM.42.12.5889-5891.2004.

Authors

Banu Sancak¹, John H Rex, Enuo Chen, Kieren Marr

Affiliation

¹ Department of Clinical Microbiology and Microbiology, Hacettepe University Medical School, Ankara 06100, Turkey. banusancak@yahoo.com

Abstract

We compared HinfI restriction endonuclease-based analysis of genomic DNA with a PCR-based method for molecular typing of 90 Candida krusei isolates from 17 geographically related patients. Strain groupings by these methods were the same for 89 of 90 isolates. Ten of 17 patients were infected with related strains of C. krusei.

Publication types

Comparative Study

MeSH terms

Candida / classification*
Candida / genetics
Candidiasis / diagnosis
Candidiasis / microbiology
Cross Infection / diagnosis
Cross Infection / microbiology
DNA, Fungal / analysis
DNA, Fungal / metabolism
Deoxyribonucleases, Type II Site-Specific / metabolism*
Humans
Mycological Typing Techniques*
Polymerase Chain Reaction / methods*
Restriction Mapping*

Substances

DNA, Fungal
Deoxyribonucleases, Type II Site-Specific
GANTC-specific type II deoxyribonucleases