Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument

Jeffrey J Germer; Jill N Vandenameele; P Shawn Mitchell; W Scott Harmsen; Joseph D C Yao

doi:10.1016/j.diagmicrobio.2003.12.003

Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument

Diagn Microbiol Infect Dis. 2004 May;49(1):59-61. doi: 10.1016/j.diagmicrobio.2003.12.003.

Authors

Jeffrey J Germer¹, Jill N Vandenameele, P Shawn Mitchell, W Scott Harmsen, Joseph D C Yao

Affiliation

¹ Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.

PMID: 15135502
DOI: 10.1016/j.diagmicrobio.2003.12.003

Abstract

Use of the MagNA Pure LC Total Nucleic Acid Isolation Kit-Large Volume in conjunction with the TRUGENE HIV-1 Genotyping Kit yielded consistent, interpretable sequence data from 1-mL plasma preparations containing HIV-1 RNA concentrations of > or =400 copies/mL. This finding was confirmed in 18 of 24 low-titer clinical specimens.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Automation
Genotype
HIV-1 / genetics*
HIV-1 / isolation & purification
Humans
RNA, Viral / analysis
Reagent Kits, Diagnostic*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sampling Studies
Sensitivity and Specificity

Substances

RNA, Viral
Reagent Kits, Diagnostic