Genetic analysis of an ambler class A extended-spectrum beta-lactamase from Capnocytophaga ochracea

J Clin Microbiol. 2004 Feb;42(2):888-90. doi: 10.1128/JCM.42.2.888-890.2004.

Abstract

A beta-lactamase gene (cfxA3, 966 bp) was isolated from a beta-lactam-resistant Capnocytophaga ochracea clinical isolate and amplified using primers from the cfxA gene of Bacteroides vulgatus. The MICs of third-generation cephalosporins were much higher than those of the transconjugant Escherichia coli strain. The deduced protein sequence, by comparison with CfxA2 of Prevotella intermedia, had a Y239D substitution and possessed the characteristics of a class A, group 2e beta-lactamase.

MeSH terms

  • Amino Acid Substitution
  • Base Sequence
  • DNA Primers
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / genetics*
  • Gram-Negative Bacteria / isolation & purification
  • Humans
  • Immunocompromised Host
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Restriction Mapping
  • Sepsis / epidemiology
  • Sepsis / microbiology
  • beta-Lactamases / analysis
  • beta-Lactamases / genetics*

Substances

  • DNA Primers
  • cfxA protein, Prevotella
  • beta-Lactamases

Associated data

  • GENBANK/U38243