Identification of tissue-embedded ascarid larvae by ribosomal DNA sequencing

Kenji Ishiwata; Akio Shinohara; Kinpei Yagi; Yoichiro Horii; Kimiyuki Tsuchiya; Yukifumi Nawa

doi:10.1007/s00436-003-1010-7

Identification of tissue-embedded ascarid larvae by ribosomal DNA sequencing

Parasitol Res. 2004 Jan;92(1):50-2. doi: 10.1007/s00436-003-1010-7. Epub 2003 Nov 4.

Authors

Kenji Ishiwata¹, Akio Shinohara, Kinpei Yagi, Yoichiro Horii, Kimiyuki Tsuchiya, Yukifumi Nawa

Affiliation

¹ Department of Parasitology, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889-1692, Japan. kishiwata@jikei.ac.jp

PMID: 14598166
DOI: 10.1007/s00436-003-1010-7

Abstract

Polymerase chain reaction (PCR) was applied to identify tissue-embedded ascarid nematode larvae. Two sequences of the internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA), ITS1 and ITS2, of the ascarid parasites were amplified and compared with those of ascarid-nematodes registered in a DNA database (GenBank). The ITS sequences of the PCR products obtained from the ascarid parasite specimen in our laboratory were compatible with those of registered adult Ascaris and Toxocara parasites. PCR amplification of the ITS regions was sensitive enough to detect a single larva of Ascaris suum mixed with porcine liver tissue. Using this method, ascarid larvae embedded in the liver of a naturally infected turkey were identified as Toxocara canis. These results suggest that even a single larva embedded in tissues from patients with larva migrans could be identified by sequencing the ITS regions.

Publication types

Evaluation Study

MeSH terms

Animals
Ascaridida Infections / parasitology*
Ascaridida Infections / veterinary
Ascaridoidea / classification*
Ascaridoidea / genetics
Ascaridoidea / growth & development*
Ascaris lumbricoides / classification
Ascaris lumbricoides / genetics
Ascaris suum / classification
Ascaris suum / genetics
Cat Diseases / parasitology
Cats
DNA, Helminth / analysis
DNA, Ribosomal Spacer / genetics*
Dog Diseases / parasitology
Dogs
Humans
Larva / classification
Larva / genetics
Liver / parasitology*
Molecular Sequence Data
Sequence Analysis, DNA*
Swine
Swine Diseases / parasitology
Toxocara / classification
Toxocara / genetics
Toxocara canis / classification
Toxocara canis / genetics
Turkeys / parasitology

Substances

DNA, Helminth
DNA, Ribosomal Spacer

Associated data

GENBANK/AB110019
GENBANK/AB110020
GENBANK/AB110021
GENBANK/AB110022
GENBANK/AB110023
GENBANK/AB110024
GENBANK/AB110025
GENBANK/AB110026
GENBANK/AB110027
GENBANK/AB110028
GENBANK/AB110029
GENBANK/AB110030
GENBANK/AB110031
GENBANK/AB110032
GENBANK/AB110033
GENBANK/AB110034