Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China

Y Guan; B J Zheng; Y Q He; X L Liu; Z X Zhuang; C L Cheung; S W Luo; P H Li; L J Zhang; Y J Guan; K M Butt; K L Wong; K W Chan; W Lim; K F Shortridge; K Y Yuen; J S M Peiris; L L M Poon

doi:10.1126/science.1087139

Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China

Science. 2003 Oct 10;302(5643):276-8. doi: 10.1126/science.1087139. Epub 2003 Sep 4.

Authors

Y Guan¹, B J Zheng, Y Q He, X L Liu, Z X Zhuang, C L Cheung, S W Luo, P H Li, L J Zhang, Y J Guan, K M Butt, K L Wong, K W Chan, W Lim, K F Shortridge, K Y Yuen, J S M Peiris, L L M Poon

Affiliation

¹ Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China. yguan@hkucc.hku.hk

PMID: 12958366
DOI: 10.1126/science.1087139

Abstract

A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome (SARS). SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market. All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates. The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Animals, Wild / virology*
Antibodies, Viral / blood
Blotting, Western
Carnivora / virology*
China
Coronavirus / classification
Coronavirus / genetics
Coronavirus / immunology
Coronavirus / isolation & purification*
Coronavirus Infections / veterinary
Coronavirus Infections / virology
Disease Reservoirs
Feces / virology
Genome, Viral
Humans
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / genetics
Molecular Sequence Data
Neutralization Tests
Nose / virology
Open Reading Frames / genetics
Phylogeny
Polymorphism, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Sequence Deletion
Sequence Homology, Nucleic Acid
Severe acute respiratory syndrome-related coronavirus / classification
Severe acute respiratory syndrome-related coronavirus / genetics
Severe acute respiratory syndrome-related coronavirus / immunology
Severe acute respiratory syndrome-related coronavirus / isolation & purification*
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins / chemistry
Viral Envelope Proteins / genetics
Viral Proteins / chemistry
Viral Proteins / genetics

Substances

Antibodies, Viral
Membrane Glycoproteins
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins
Viral Proteins
spike glycoprotein, SARS-CoV

Associated data

GENBANK/AY304486
GENBANK/AY304487
GENBANK/AY304488
GENBANK/AY304489