Abstract
HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD / physiology
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Cell Line
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Chimera / genetics
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Chimera / immunology
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HIV / genetics
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HIV / pathogenicity
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HIV / physiology
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HeLa Cells
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Hepacivirus / genetics
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Hepacivirus / pathogenicity*
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Hepacivirus / physiology
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Hepatitis C Antibodies
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Hepatocytes / virology
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Humans
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Hydrogen-Ion Concentration
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / physiology
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Neutralization Tests
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Receptors, Virus / antagonists & inhibitors
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Receptors, Virus / physiology
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Tetraspanin 28
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
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Viral Envelope Proteins / physiology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD81 protein, human
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E1 protein, Hepatitis C virus
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Hepatitis C Antibodies
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Membrane Proteins
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Receptors, Virus
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Tetraspanin 28
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Viral Envelope Proteins
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glycoprotein E2, Hepatitis C virus