In vitro pharmacodynamic properties of three antifungal agents against preformed Candida albicans biofilms determined by time-kill studies

Gordon Ramage; Kacy VandeWalle; Stefano P Bachmann; Brian L Wickes; José L López-Ribot

doi:10.1128/AAC.46.11.3634-3636.2002

In vitro pharmacodynamic properties of three antifungal agents against preformed Candida albicans biofilms determined by time-kill studies

Antimicrob Agents Chemother. 2002 Nov;46(11):3634-6. doi: 10.1128/AAC.46.11.3634-3636.2002.

Authors

Gordon Ramage¹, Kacy VandeWalle, Stefano P Bachmann, Brian L Wickes, José L López-Ribot

Affiliation

¹ Department of Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA.

Abstract

We have examined the in vitro activities of fluconazole, amphotericin B, and caspofungin against Candida albicans biofilms by time-kill methodology. Fluconazole was ineffective against biofilms. Killing of biofilm cells was suboptimal at therapeutic concentrations of amphotericin B. Caspofungin displayed the most effective pharmacokinetic properties, with > or =99% killing at physiological concentrations.

Publication types

Comparative Study

MeSH terms

Amphotericin B / pharmacology*
Anti-Bacterial Agents / pharmacology*
Antifungal Agents / pharmacology*
Biofilms
Candida albicans / drug effects*
Caspofungin
Colony Count, Microbial
Colorimetry
Coloring Agents
Echinocandins
Fluconazole / pharmacology*
Indicators and Reagents
Lipopeptides
Peptides*
Peptides, Cyclic*
Reproducibility of Results
Tetrazolium Salts
Time Factors

Substances

Anti-Bacterial Agents
Antifungal Agents
Coloring Agents
Echinocandins
Indicators and Reagents
Lipopeptides
Peptides
Peptides, Cyclic
Tetrazolium Salts
2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
Amphotericin B
Fluconazole
Caspofungin