Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method

J Clin Microbiol. 2000 Nov;38(11):4228-32. doi: 10.1128/JCM.38.11.4228-4232.2000.

Abstract

The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta-lactamases were enlarged by </=1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins*
  • Cefpodoxime
  • Ceftazidime / pharmacology
  • Ceftizoxime / analogs & derivatives*
  • Ceftizoxime / pharmacology*
  • Cephalosporins / pharmacology*
  • Clavulanic Acid / pharmacology
  • Humans
  • Klebsiella / drug effects
  • Klebsiella / enzymology*
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Microbial Sensitivity Tests / methods
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • Cephalosporins
  • Clavulanic Acid
  • Ceftazidime
  • Ceftizoxime
  • AmpC beta-lactamases
  • beta-Lactamases