When freshly passed in stools, the oocyst is not infective (thus, direct fecal-oral transmission cannot occur; this differentiates Cyclospora from another important coccidian parasite, Cryptosporidium).  In the environment , sporulation occurs after days or weeks at temperatures between 22°C to 32°C, resulting in division of the sporont into two sporocysts, each containing two elongate sporozoites .  Fresh produce and water can serve as vehicles for transmission and the sporulated oocysts are ingested (in contaminated food or water) .  The oocysts excyst in the gastrointestinal tract, freeing the sporozoites which invade the epithelial cells of the small intestine .  Inside the cells they undergo asexual multiplication and sexual development to mature into oocysts, which will be shed in stools .  The potential mechanisms of contamination of food and water are still under investigation.